Hotspot

rpl32-trnL-UAG

SSC · 114744-115850 · 1107 bp

Back to Herpetospermum

Pi 0.0669

MarkerSeek score 79.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1107
variable_sites: 71
pi: 0.0669
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTGGAAAAACAAAGGGCGTC	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.4	58.0	1242-1274 bp	1.0	82.9
rpl32-trnL-UAG_p2	TTGGAAAAACAAAGGGCGTC	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.4	58.0	1243-1275 bp	1.0	82.9
rpl32-trnL-UAG_p3	TTGGAAAAACAAAGGGCGTC	GGAGAGCGGGGACTAATAGT	45.0	55.0	57.4	58.0	1797-1830 bp	1.0	81.1
rpl32-trnL-UAG_p4	CGAAAGCTTTTTCGCTAGCA	GGAGAGCGGGGACTAATAGT	45.0	55.0	57.4	58.0	1768-1801 bp	1.0	81.1
rpl32-trnL-UAG_p5	TTGGAAAAACAAAGGGCGTC	TGGAGAGCGGGGACTAATAG	45.0	55.0	57.4	58.0	1798-1831 bp	1.0	81.0