Hotspot

atpB-rbcL

LSC · 55700-56471 · 772 bp

Back to Herpetospermum

Pi 0.0301
MarkerSeek score 69.70
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
772
variable_sites
23
pi
0.0301
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CGATACGTCCCGAGTTGTTT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.0 58.0 891-911 bp 1.0 74.6
atpB-rbcL_p2 CGATACGTCCCGAGTTGTTT ACCAGCTTTGAATCCAACACT 50.0 42.9 58.0 58.1 890-910 bp 1.0 74.3
atpB-rbcL_p3 TGATTTGAGCGATACGTCCC CACCAGCTTTGAATCCAACAC 50.0 47.6 57.8 58.0 900-920 bp 1.0 72.9
atpB-rbcL_p4 TGATTTGAGCGATACGTCCC ACCAGCTTTGAATCCAACACT 50.0 42.9 57.8 58.1 899-919 bp 1.0 72.7
atpB-rbcL_p5 CGATACGTCCCGAGTTGTTT ACACCAGCTTTGAATCCAACA 50.0 42.9 58.0 58.3 892-912 bp 1.0 72.1