Hotspot

psaA-ycf3

LSC · 42977-43703 · 727 bp

Back to Herpetospermum

Pi 0.0246

MarkerSeek score 77.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 17
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTCGAGAAATGACCCGG	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.4	58.0	898-929 bp	1.0	81.4
psaA-ycf3_p2	TGTTCTCGAGAAATGACCCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.6	58.0	899-930 bp	1.0	81.3
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	827-858 bp	1.0	80.7
psaA-ycf3_p4	GTTCTCGAGAAATGACCCGG	AGCCGCTGAGTATTGGAAAC	55.0	50.0	58.4	58.3	894-925 bp	1.0	79.6
psaA-ycf3_p5	GTTCTCGAGAAATGACCCGG	CAAGCCGCTGAGTATTGGAA	55.0	50.0	58.4	58.3	896-927 bp	1.0	79.6