Hotspot

atpA-atpF

LSC · 11782-11846 · 65 bp

Back to Herpetospermum

Pi 0.0484

MarkerSeek score 73.30

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 3
pi: 0.0484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CATCTGCTCGAATGGTTACCA	ATTGGCATGTTTGGGACGAT	47.6	45.0	58.1	58.1	123-126 bp	1.0	73.9
atpA-atpF_p2	TCATCTGCTCGAATGGTTACC	ATTGGCATGTTTGGGACGAT	47.6	45.0	57.8	58.1	124-127 bp	1.0	73.2
atpA-atpF_p3	ATCTGCTCGAATGGTTACCA	ATTGGCATGTTTGGGACGAT	45.0	45.0	56.6	58.1	122-125 bp	1.0	71.7
atpA-atpF_p4	CATCTGCTCGAATGGTTACCA	TATTGGCATGTTTGGGACGA	47.6	45.0	58.1	57.5	124-127 bp	1.0	71.1
atpA-atpF_p5	TCATCTGCTCGAATGGTTACC	TATTGGCATGTTTGGGACGA	47.6	45.0	57.8	57.5	125-128 bp	1.0	70.4