Hotspot

trnR-UCU-atpA

LSC · 9708-10257 · 550 bp

Back to Herpetospermum

Pi 0.0523

MarkerSeek score 74.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 550
variable_sites: 28
pi: 0.0523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ACTAGAGACGTCGACATCGT	CCGCAGCTCAAATTAAAGCC	50.0	50.0	58.0	58.1	1659-1668 bp	1.0	83.9
trnR-UCU-atpA_p2	ACTAGAGACGTCGACATCGT	GCCGGCAAATCAAAATTGGA	50.0	45.0	58.0	57.9	1627-1636 bp	1.0	83.9
trnR-UCU-atpA_p3	ACTAGAGACGTCGACATCGT	ACAAGTAGCCGGCAAATCAA	50.0	45.0	58.0	58.1	1634-1643 bp	1.0	83.9
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	GCTTATCCGGGGGATGTTTT	50.0	50.0	58.0	57.9	1581-1587 bp	1.0	83.7
trnR-UCU-atpA_p5	ACTAGAGACGTCGACATCGT	GCTTATCCGGGGGATGTTTT	50.0	50.0	58.0	57.9	1909-1918 bp	1.0	83.5