Hotspot

trnS-GCU-trnG-UCC

LSC · 7845-9312 · 1468 bp

Back to Herpetospermum

Pi 0.0316

MarkerSeek score 68.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1468
variable_sites: 46
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1550-1581 bp	1.0	82.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1549-1580 bp	1.0	82.8
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.0	58.1	1547-1578 bp	1.0	82.6
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.0	58.1	1546-1577 bp	1.0	82.6
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.2	58.0	1604-1635 bp	1.0	81.5