Hotspot

trnK-UUU-rps16

LSC · 4215-4807 · 593 bp

Back to Herpetospermum

Pi 0.0517

MarkerSeek score 74.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 30
pi: 0.0517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	ACACATTTCCCTCTACCCCA	CCCAATGAGCCGTTTATCGA	50.0	50.0	57.9	58.1	1282-1332 bp	1.0	82.7
trnK-UUU-rps16_p2	ACACATTTCCCTCTACCCCA	TCCCAATGAGCCGTTTATCG	50.0	50.0	57.9	58.1	1283-1333 bp	1.0	82.7
trnK-UUU-rps16_p3	ACACATTTCCCTCTACCCCA	ACAAGGCAGGGGTTTTTAGG	50.0	50.0	57.9	58.0	1102 bp	0.5	59.5
trnK-UUU-rps16_p4	AGTCGCGGTCTTACAAACTC	ACAAGGCAGGGGTTTTTAGG	50.0	50.0	57.9	58.0	751 bp	0.5	59.4
trnK-UUU-rps16_p5	GTCGCGGTCTTACAAACTCT	ACAAGGCAGGGGTTTTTAGG	50.0	50.0	57.9	58.0	750 bp	0.5	59.4