Hotspot

trnH-GUG-psbA

LSC · 116-286 · 171 bp

Back to Herpetospermum

Pi 0.0435

MarkerSeek score 73.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 7
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGCTGCTGTTGATGTTCCAT	55.0	45.0	58.1	57.8	220-227 bp	1.0	83.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AGCTGCTGTTGATGTTCCAT	50.0	45.0	57.9	57.8	234-241 bp	1.0	83.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AGCTGCTGTTGATGTTCCAT	50.0	45.0	57.9	57.8	233-240 bp	1.0	83.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGCTGCTGTTGATGTTCCAT	50.0	45.0	57.9	57.8	235-242 bp	1.0	82.9
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	GCTGCTGTTGATGTTCCATC	55.0	50.0	58.1	57.4	219-226 bp	1.0	80.6