Hotspot

rps16

LSC · 5188-6346 · 1159 bp

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Pi 0.00608

MarkerSeek score 61.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1159
variable_sites: 13
pi: 0.00608
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGTCATCCACACCCTCTT	TGTATCGACTGAACCAATGACT	50.0	40.9	58.0	57.2	1252-1260 bp	1.0	58.8
rps16_p2	ATGAGTCATCCACACCCTCT	TGTATCGACTGAACCAATGACT	50.0	40.9	57.7	57.2	1253-1261 bp	1.0	57.0
rps16_p3	TGAGTCATCCACACCCTCTT	TGTATCGACTGAACCAATGAC	50.0	42.9	58.0	55.7	1252-1260 bp	1.0	55.1
rps16_p4	TGAGTCATCCACACCCTCTT	ATGTATCGACTGAACCAATGACT	50.0	39.1	58.0	57.7	1253-1261 bp	1.0	54.5
rps16_p5	TGAGTCATCCACACCCTCTT	TCGACTGAACCAATGACTATTCA	50.0	39.1	58.0	57.6	1248-1256 bp	1.0	54.0