Hotspot

ndhA

IRa · 133678-135907 · 2230 bp

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Pi 0.00628

MarkerSeek score 48.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: IRa
length_bp: 2230
variable_sites: 27
pi: 0.00628
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTACTCGGACTGTTTGGTGG	TCGGTGAAATGACGGAATCC	55.0	50.0	57.9	58.0	2741-2757 bp	1.0	80.6
ndhA_p2	GGACTGTTTGGTGGCCATAA	TCGGTGAAATGACGGAATCC	50.0	50.0	58.1	58.0	2735-2751 bp	1.0	80.6
ndhA_p3	CTACTCGGACTGTTTGGTGG	GTTTATCCGGCCCAATGCTA	55.0	50.0	57.9	58.0	2882-2898 bp	1.0	80.5
ndhA_p4	CTACTCGGACTGTTTGGTGG	TTTATCCGGCCCAATGCTAC	55.0	50.0	57.9	58.0	2881-2897 bp	1.0	80.5
ndhA_p5	GGACTGTTTGGTGGCCATAA	TTTATCCGGCCCAATGCTAC	50.0	50.0	58.1	58.0	2875-2891 bp	1.0	80.5