Hotspot

clpP

LSC · 74644-76840 · 2197 bp

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Pi 0.0121

MarkerSeek score 61.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2197
variable_sites: 48
pi: 0.0121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.8	57.7	2266-2286 bp	1.0	64.4
clpP_p2	GGATTGGTGTTAAATCGTTGCT	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.6	57.7	2265-2285 bp	1.0	62.3
clpP_p3	GGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	42.9	45.0	56.1	57.7	2265-2285 bp	1.0	58.8
clpP_p4	GGTGTTAAATCGTTGCTATTGCT	ACGCTTCCACATCAAAGTGA	39.1	45.0	58.0	57.7	2260-2280 bp	1.0	57.6
clpP_p5	TGTGGATTGGTGTTAAATCGTTG	ACGCTTCCACATCAAAGTGA	39.1	45.0	57.9	57.7	2268-2288 bp	1.0	56.8