Hotspot

ndhG-ndhI

SSC · 132672-133091 · 420 bp

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Pi 0.0155

MarkerSeek score 52.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 420
variable_sites: 13
pi: 0.0155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATTGGTCCAGGCAAATCCAT	GCCGTTTACCGATGTCAGTA	45.0	50.0	57.4	57.7	516-517 bp	1.0	75.5
ndhG-ndhI_p2	TATTGGTCCAGGCAAATCCA	GCCGTTTACCGATGTCAGTA	45.0	50.0	56.8	57.7	517-518 bp	1.0	70.2
ndhG-ndhI_p3	GTATTGGTCCAGGCAAATCCA	GCCGTTTACCGATGTCAGTA	47.6	50.0	58.3	57.7	518-519 bp	1.0	69.8
ndhG-ndhI_p4	TGTATTGGTCCAGGCAAATCC	GCCGTTTACCGATGTCAGTA	47.6	50.0	58.3	57.7	519-520 bp	1.0	69.8
ndhG-ndhI_p5	ATTGGTCCAGGCAAATCCAT	GCCGTTTACCGATGTCAGT	45.0	52.6	57.4	57.9	516-517 bp	1.0	68.7