Hotspot

ndhF-rpl32

SSC · 125719-126775 · 1057 bp

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Pi 0.0117
MarkerSeek score 57.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1057
variable_sites
24
pi
0.0117
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCGTCGGAAAAAGTA GGTTGAAAGCAAGGGAAGGA 45.0 50.0 58.0 58.0 1625-1692 bp 1.0 83.5
ndhF-rpl32_p2 CGGAAAAAGTAGGAGTCCCG GGTTGAAAGCAAGGGAAGGA 55.0 50.0 58.0 58.0 1616-1658 bp 0.75 72.3
ndhF-rpl32_p3 CGGAAAAAGTAGGAGTCCCG GGGGGTTTTCTCAATTGGGT 55.0 50.0 58.0 58.0 1633-1675 bp 0.75 72.2
ndhF-rpl32_p4 TAGGAATGGGAAGTGGCAGA GGTTGAAAGCAAGGGAAGGA 50.0 50.0 58.0 58.0 1584-1626 bp 0.75 72.2
ndhF-rpl32_p5 TAGGAATGGGAAGTGGCAGA GGGGGTTTTCTCAATTGGGT 50.0 50.0 58.0 58.0 1601-1643 bp 0.75 72.0