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rpl36-rps8

LSC · 84565-85042 · 478 bp

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Pi 0.0119

MarkerSeek score 61.00

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 11
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTTACGAACGGAAGCCCTT	GCGGGATGGGGATTGTAATT	45.0	50.0	57.7	58.0	602-611 bp	1.0	80.4
rpl36-rps8_p2	CCCTCCTACGGATTAGTCGA	GCGGGATGGGGATTGTAATT	55.0	50.0	57.7	58.0	635-644 bp	1.0	80.2
rpl36-rps8_p3	TAATTCGTCCCCTCCTACGG	GCGGGATGGGGATTGTAATT	55.0	50.0	58.3	58.0	644-653 bp	1.0	79.9
rpl36-rps8_p4	GTCCCCTCCTACGGATTAGT	GCGGGATGGGGATTGTAATT	55.0	50.0	57.6	58.0	638-647 bp	1.0	79.3
rpl36-rps8_p5	ACGAACGGAAGCCCTTATTT	GCGGGATGGGGATTGTAATT	45.0	50.0	57.5	58.0	598-607 bp	1.0	78.6