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psaJ-rpl33

LSC · 71490-72006 · 517 bp

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Pi 0.0164

MarkerSeek score 54.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 517
variable_sites: 17
pi: 0.0164
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.1	58.2	1170-1175 bp	1.0	79.2
psaJ-rpl33_p2	CCCGTGACATTTTGTACCCA	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.1	58.2	1169-1174 bp	1.0	79.2
psaJ-rpl33_p3	ACAGGATTTGAACCCGTGAC	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.1	58.2	1181-1186 bp	1.0	79.1
psaJ-rpl33_p4	GGATGCGTTAACATTCCCCT	TCAATTCGATCCCCCGATTG	50.0	50.0	57.9	57.7	1235-1239 bp	0.75	68.1
psaJ-rpl33_p5	GGATGCGTTAACATTCCCCT	ATACCTGGAAATGCCCGTTG	50.0	50.0	57.9	58.2	644 bp	0.75	67.9