Hotspot

atpB-rbcL

LSC · 58122-59017 · 896 bp

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Pi 0.00839

MarkerSeek score 65.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 15
pi: 0.00839
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.0	58.1	974-982 bp	1.0	80.6
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.0	58.4	975-983 bp	1.0	78.2
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	GTGGTGACATAAGTCCCTCC	45.0	55.0	58.0	57.3	976-984 bp	1.0	76.0
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	AAGTCCCTCCCTACAACTCA	45.0	50.0	58.0	57.3	966-974 bp	1.0	75.9
atpB-rbcL_p5	TTTCAAGCGTGGAAACTCCA	AGTCCCTCCCTACAACTCAT	45.0	50.0	58.0	57.0	965-973 bp	1.0	74.0