Hotspot

rps4-trnT-UGU

LSC · 48694-49166 · 473 bp

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Pi 0.0074

MarkerSeek score 61.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 7
pi: 0.0074
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	633-704 bp	1.0	79.7
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	637-708 bp	1.0	79.4
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	601-672 bp	1.0	78.9
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	605-676 bp	1.0	78.6
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	641-712 bp	1.0	76.8