Hotspot

psbZ-trnG-UCC

LSC · 37949-38780 · 832 bp

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Pi 0.0222

MarkerSeek score 66.10

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 34
pi: 0.0222
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GGTATCCTTAATTCTCTTATTTCTTGA	TACCATTCGACCATATCCGC	29.6	50.0	55.3	57.3	831-886 bp	1.0	47.2
psbZ-trnG-UCC_p2	GGTATCCTTAATTCTCTTATTTCTTG	TACCATTCGACCATATCCGC	30.8	50.0	54.0	57.3	831-886 bp	1.0	47.2
psbZ-trnG-UCC_p3	GGTATCCTTAATTCTCTTATTTCTTGA	CGTCTTCTCCTTGGCAAAGA	29.6	50.0	55.3	57.8	858-913 bp	1.0	47.1
psbZ-trnG-UCC_p4	GGTATCCTTAATTCTCTTATTTCTTG	CGTCTTCTCCTTGGCAAAGA	30.8	50.0	54.0	57.8	858-913 bp	1.0	47.1
psbZ-trnG-UCC_p5	GGTATCCTTAATTCTCTTATTTCTT	CGTCTTCTCCTTGGCAAAGA	28.0	50.0	52.6	57.8	858-913 bp	1.0	47.1