Hotspot

ccsA

Genome · 117298-118272 · 975 bp

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Pi 0.00519

MarkerSeek score 41.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 975
variable_sites: 14
pi: 0.00519
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AACCGACAAAAGCCTGGAAT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	1895-1942 bp	1.0	84.2
ccsA_p2	ACCGACAAAAGCCTGGAATT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	1894-1941 bp	1.0	84.2
ccsA_p3	AACCGACAAAAGCCTGGAAT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	1894-1941 bp	1.0	84.2
ccsA_p4	ACCGACAAAAGCCTGGAATT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	1893-1940 bp	1.0	84.2
ccsA_p5	GTGAAATCGGTAGACACGCT	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.0	58.0	1498-1522 bp	1.0	82.9