Hotspot

rpl32-trnL-UAG

Genome · 116202-117121 · 920 bp

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Pi 0.0158
MarkerSeek score 62.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
920
variable_sites
34
pi
0.0158
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 992-1044 bp 1.0 84.1
rpl32-trnL-UAG_p2 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 993-1045 bp 1.0 84.1
rpl32-trnL-UAG_p3 GCAGCCTTAAAAGCGTTGTC CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.3 58.1 1013-1065 bp 1.0 83.5
rpl32-trnL-UAG_p4 GCAGCCTTAAAAGCGTTGTC CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.3 58.1 1015-1067 bp 1.0 83.5
rpl32-trnL-UAG_p5 GCAGCCTTAAAAGCGTTGTC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.3 57.9 1002-1054 bp 1.0 83.1