Hotspot

ndhF-rpl32

Genome · 115072-116039 · 968 bp

Back to Heracleum

Pi 0.0202

MarkerSeek score 67.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 968
variable_sites: 45
pi: 0.0202
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TCCAAATATTTTTACGAATACGCT	50.0	29.2	58.0	55.3	1083-1150 bp	1.0	47.2
ndhF-rpl32_p2	TGCCGACGGAAAAAGAAGAA	TCCAAATATTTTTACGAATACGCT	45.0	29.2	58.0	55.3	1081-1148 bp	1.0	47.2
ndhF-rpl32_p3	TTGCCGACGGAAAAAGAAGA	TCCAAATATTTTTACGAATACGCT	45.0	29.2	58.0	55.3	1082-1149 bp	1.0	47.2
ndhF-rpl32_p4	AAAAGAAGAAGTCCCGCTCC	TCCAAATATTTTTACGAATACGCT	50.0	29.2	57.8	55.3	1071-1138 bp	1.0	47.2
ndhF-rpl32_p5	TTTGTTGCCGACGGAAAAAG	TCCAAATATTTTTACGAATACGCT	45.0	29.2	57.8	55.3	1086-1153 bp	1.0	47.2