Hotspot

ycf1-ndhF

Genome · 112803-112833 · 31 bp

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Pi 0.121

MarkerSeek score 62.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: Genome
length_bp: 31
variable_sites: 9
pi: 0.121
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CCCTGCCCACACTAGAAAAA	GTTGGATCCCTCGGAATTCC	50.0	55.0	57.7	58.0	1244-1310 bp	1.0	85.0
ycf1-ndhF_p2	TACGTATCCGCCTAGCTTGT	GTTGGATCCCTCGGAATTCC	50.0	55.0	58.3	58.0	1297-1363 bp	1.0	84.4
ycf1-ndhF_p3	CCCTGCCCACACTAGAAAAA	TGGATCCCTCGGAATTCCTT	50.0	50.0	57.7	58.1	1242-1308 bp	1.0	84.4
ycf1-ndhF_p4	CCCTGCCCACACTAGAAAAA	TTGGATCCCTCGGAATTCCT	50.0	50.0	57.7	58.1	1243-1309 bp	1.0	84.4
ycf1-ndhF_p5	TACGTATCCGCCTAGCTTGT	TGGATCCCTCGGAATTCCTT	50.0	50.0	58.3	58.1	1295-1361 bp	1.0	83.9