Hotspot

trnV-GAC-rrn16

Genome · 100524-102293 · 1770 bp

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Pi 0.0219

MarkerSeek score 52.00

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 1770
variable_sites: 75
pi: 0.0219
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	GGAAGTCATCAGTTCGAGCC	CCGACTTGCATGTGTTAAGC	55.0	50.0	58.4	57.8	325-2287 bp	1.0	83.9
trnV-GAC-rrn16_p2	TGGAAGTCATCAGTTCGAGC	CCGACTTGCATGTGTTAAGC	50.0	50.0	57.6	57.8	326-2288 bp	1.0	83.7
trnV-GAC-rrn16_p3	AAGTCATCAGTTCGAGCCTG	CCGACTTGCATGTGTTAAGC	50.0	50.0	57.6	57.8	323-2285 bp	1.0	83.7
trnV-GAC-rrn16_p4	GGAAGTCATCAGTTCGAGCC	GCAGGTTCTTACGCGTTACT	55.0	50.0	58.4	58.3	378-2340 bp	1.0	83.3
trnV-GAC-rrn16_p5	TGGAAGTCATCAGTTCGAGC	GCAGGTTCTTACGCGTTACT	50.0	50.0	57.6	58.3	379-2341 bp	1.0	83.2