Hotspot

ycf2-trnL-CAA

Genome · 93479-94110 · 632 bp

Back to Heracleum

Pi 0.108

MarkerSeek score 84.20

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: Genome
length_bp: 632
variable_sites: 128
pi: 0.108
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	TAGCCGGAAAGTTATGTGGC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	723-2811 bp	1.0	86.6
ycf2-trnL-CAA_p2	TAGCCGGAAAGTTATGTGGC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	725-2813 bp	1.0	86.6
ycf2-trnL-CAA_p3	TAGCCGGAAAGTTATGTGGC	GCCTTGGTGGTGAAATGGTA	50.0	50.0	58.0	58.1	735-2823 bp	1.0	86.0
ycf2-trnL-CAA_p4	TAGCCGGAAAGTTATGTGGC	TGGTGAAATGGTAGACACGC	50.0	50.0	58.0	58.2	728-2816 bp	1.0	85.1
ycf2-trnL-CAA_p5	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	785-2873 bp	1.0	84.8