Hotspot

psbA-trnK-UUU

Genome · 1330-1530 · 201 bp

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Pi 0.0374

MarkerSeek score 62.40

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: Genome
length_bp: 201
variable_sites: 15
pi: 0.0374
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	313-322 bp	1.0	82.8
psbA-trnK-UUU_p2	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.7	57.8	314-323 bp	1.0	82.3
psbA-trnK-UUU_p3	TACAGAAGCGACCCCATAGG	CTAGTTCCGGGTTCGAATCC	55.0	55.0	58.6	57.8	282-291 bp	1.0	80.8
psbA-trnK-UUU_p4	TTTTCGGTGCTGGTTATCCA	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.4	57.8	304-313 bp	1.0	80.4
psbA-trnK-UUU_p5	TAAAGACGGTTTTCGGTGCT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.8	58.6	324-333 bp	1.0	79.5