Hotspot

petB

LSC · 77337-78768 · 1432 bp

Back to Heptapleurum

Pi 0.00886

MarkerSeek score 40.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1432
variable_sites: 33
pi: 0.00886
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GTGTATTTCTAGCCCAGCCC	ACTTTCTGGGTCGTCTCAGA	55.0	50.0	58.0	58.0	2172-2173 bp	0.857143	75.5
petB_p2	GTGTATTTCTAGCCCAGCCC	TTCAAGCGAACCAGCCTATC	55.0	50.0	58.0	58.0	1834-1835 bp	0.857143	75.4
petB_p3	GTGTATTTCTAGCCCAGCCC	CTTCAAGCGAACCAGCCTAT	55.0	50.0	58.0	58.0	1835-1836 bp	0.857143	75.4
petB_p4	TCCTTTGATGGGTGTTGCAA	ACTTTCTGGGTCGTCTCAGA	45.0	50.0	57.9	58.0	2331 bp	0.071429	42.2
petB_p5	TCCTTTGATGGGTGTTGCAA	TTCAAGCGAACCAGCCTATC	45.0	50.0	57.9	58.0	1993 bp	0.071429	42.0