Hotspot

ndhF-rpl32

SSC · 114789-115986 · 1198 bp

Back to Heptapleurum

Pi 0.00744

MarkerSeek score 50.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1198
variable_sites: 38
pi: 0.00744
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1362-1383 bp	1.0	82.3
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1360-1381 bp	1.0	82.2
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1361-1382 bp	1.0	82.2
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1373-1394 bp	1.0	81.1
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	GACAACGCTTTTAAGGCTGC	45.0	50.0	58.0	58.3	1371-1392 bp	1.0	81.0