Hotspot

rpl20-rps12

LSC · 71365-72139 · 775 bp

Back to Heptapleurum

Pi 0.00556
MarkerSeek score 50.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
775
variable_sites
17
pi
0.00556
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCTACGTCGCCGAGCTATAT CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 846-848 bp 1.0 75.3
rpl20-rps12_p2 CTTGAATGAGCCCCTCGAAA CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 893-895 bp 1.0 75.0
rpl20-rps12_p3 TTGAATGAGCCCCTCGAAAG CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 892-894 bp 1.0 75.0
rpl20-rps12_p4 TTCTACGTCGCCGAGCTATA CAGCGTCGAGGAACATGTAC 50.0 55.0 58.1 58.7 847-849 bp 1.0 75.0
rpl20-rps12_p5 TTTTTGTTCTACGTCGCCGA CAGCGTCGAGGAACATGTAC 45.0 55.0 58.1 58.7 853-855 bp 1.0 74.8