Hotspot

ndhC-trnM-CAT

LSC · 52936-54783 · 1848 bp

Back to Heptapleurum

Pi 0.00787

MarkerSeek score 52.40

Primer available yes

Label ndhC-trnM-CAT

Feature Metadata

label_name: ndhC-trnM-CAT
feature_type: igs
region: LSC
length_bp: 1848
variable_sites: 51
pi: 0.00787
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAT_p1	CTCCGGAAATGAAAAATGCCA	CCCGCCGTATGAAAGCAATA	42.9	50.0	57.4	58.4	1888-1993 bp	1.0	70.1
ndhC-trnM-CAT_p2	ACTCCGGAAATGAAAAATGCC	CCCGCCGTATGAAAGCAATA	42.9	50.0	57.4	58.4	1889-1994 bp	1.0	70.1
ndhC-trnM-CAT_p3	TCCGGAAATGAAAAATGCCA	CCCGCCGTATGAAAGCAATA	40.0	50.0	56.2	58.4	1887-1992 bp	1.0	68.4
ndhC-trnM-CAT_p4	CTCCGGAAATGAAAAATGCCA	CCGCCGTATGAAAGCAATAC	42.9	50.0	57.4	57.3	1887-1992 bp	1.0	68.1
ndhC-trnM-CAT_p5	ACTCCGGAAATGAAAAATGCC	CCGCCGTATGAAAGCAATAC	42.9	50.0	57.4	57.3	1888-1993 bp	1.0	68.1