Hotspot

ycf3-trnS-GGA

LSC · 46780-47638 · 859 bp

Back to Heptapleurum

Pi 0.0069

MarkerSeek score 51.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 859
variable_sites: 20
pi: 0.0069
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTGGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.3	58.1	1022-1031 bp	1.0	67.1
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTGGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.3	58.1	1023-1032 bp	1.0	67.1
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTGGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.3	58.2	1043-1052 bp	1.0	66.6
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTGGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.3	58.2	1044-1053 bp	1.0	66.6
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTGGGAA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.3	57.8	993-1002 bp	1.0	66.4