Hotspot

trnS-GCT-trnR-TCT

LSC · 9481-11155 · 1675 bp

Back to Heptapleurum

Pi 0.00958

MarkerSeek score 45.50

Primer available yes

Label trnS-GCT-trnR-TCT

Feature Metadata

label_name: trnS-GCT-trnR-TCT
feature_type: igs
region: LSC
length_bp: 1675
variable_sites: 56
pi: 0.00958
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnR-TCT_p1	ATCTAATGATCCGGGGCGTA	CGCGGTGGAAGAACAGATAA	50.0	50.0	58.1	58.0	2354-2389 bp	1.0	82.8
trnS-GCT-trnR-TCT_p2	TATCTAATGATCCGGGGCGT	CGCGGTGGAAGAACAGATAA	50.0	50.0	58.1	58.0	2355-2390 bp	1.0	82.8
trnS-GCT-trnR-TCT_p3	ATTAGCAATCCGCCGCTTTA	CGCGGTGGAAGAACAGATAA	45.0	50.0	58.0	58.0	2127-2155 bp	1.0	82.7
trnS-GCT-trnR-TCT_p4	ATTAGCAATCCGCCGCTTTA	CGTTTCCAGAGTGGGATCTG	45.0	55.0	58.0	58.0	2325-2353 bp	1.0	82.5
trnS-GCT-trnR-TCT_p5	ATCTAATGATCCGGGGCGTA	CGTTTCCAGAGTGGGATCTG	50.0	55.0	58.1	58.0	2552-2587 bp	1.0	82.5