Hotspot

rps16-trnQ-TTG

LSC · 6351-8115 · 1765 bp

Back to Heptapleurum

Pi 0.00988

MarkerSeek score 54.10

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1765
variable_sites: 61
pi: 0.00988
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1263-1838 bp	1.0	85.0
rps16-trnQ-TTG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1278-1853 bp	1.0	83.1
rps16-trnQ-TTG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1277-1852 bp	1.0	83.1
rps16-trnQ-TTG_p4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1334-1909 bp	1.0	82.0
rps16-trnQ-TTG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1267-1842 bp	1.0	80.3