Hotspot

psbA-trnK-UUU

LSC · 1545-1763 · 219 bp

Back to Heptapleurum

Pi 0.0148

MarkerSeek score 50.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 12
pi: 0.0148
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.8	57.9	415-422 bp	1.0	80.6
psbA-trnK-UUU_p2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	330-337 bp	1.0	80.6
psbA-trnK-UUU_p3	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.7	57.9	416-423 bp	1.0	80.1
psbA-trnK-UUU_p4	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.7	57.8	331-338 bp	1.0	80.1
psbA-trnK-UUU_p5	TAAAGACGGTTTTCGGTGCT	TTTGGGGAGGGGTTTTTACC	45.0	50.0	57.8	57.6	382-389 bp	1.0	78.8