Hotspot

rps16

LSC · 1159-1904 · 746 bp

Back to Hepatica

Pi 0.0067

MarkerSeek score 52.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 746
variable_sites: 17
pi: 0.0067
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TACCCTCCCTTTTGTTTGGC	ATTGGATTGCACAACCCCAT	50.0	45.0	58.0	58.0	1861-1995 bp	1.0	85.7
rps16_p2	TACCCTCCCTTTTGTTTGGC	TGTGTTGGATTGGCACTTCA	50.0	45.0	58.0	57.9	1448-1580 bp	1.0	85.5
rps16_p3	ATCTCCCCGTGAAGAATTGC	ATTGGATTGCACAACCCCAT	50.0	45.0	58.0	58.0	2044-2178 bp	1.0	85.2
rps16_p4	ATCTCCCCGTGAAGAATTGC	TGTGTTGGATTGGCACTTCA	50.0	45.0	58.0	57.9	1631-1763 bp	1.0	85.0
rps16_p5	TACCCTCCCTTTTGTTTGGC	ACTTGTGTTGGATTGGCACT	50.0	45.0	58.0	57.9	1451-1583 bp	0.888889	80.4