Hotspot

ndhG-ndhI

SSC · 119672-119831 · 160 bp

Back to Hepatica

Pi 0.0207

MarkerSeek score 65.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 160
variable_sites: 11
pi: 0.0207
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATTGGTCCAGGCAAATCCAT	GTCGGTTGCCAATGTCAGTA	45.0	50.0	57.4	58.2	292-699 bp	1.0	76.6
ndhG-ndhI_p2	ATTGGTCCAGGCAAATCCAT	TCGGTTGCCAATGTCAGTAA	45.0	45.0	57.4	57.4	291-698 bp	1.0	73.8
ndhG-ndhI_p3	ATTGGTCCAGGCAAATCCAT	AAAAAGGCACGGCTAAACCA	45.0	45.0	57.4	58.6	226-633 bp	1.0	73.7
ndhG-ndhI_p4	ATTGGTCCAGGCAAATCCAT	GCACGGCTAAACCACTTGAT	45.0	50.0	57.4	58.8	220-627 bp	1.0	71.9
ndhG-ndhI_p5	TCATGTATTGGTCCAGGCAA	GTCGGTTGCCAATGTCAGTA	45.0	50.0	56.8	58.2	298-705 bp	1.0	71.5