Hotspot

ndhE-ndhG

SSC · 118873-119137 · 265 bp

Back to Hepatica

Pi 0.0188

MarkerSeek score 61.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 265
variable_sites: 17
pi: 0.0188
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACAAGTACATGTTCGAGCATCA	AGTTGCCTTGATAGGTGCAA	40.9	45.0	58.1	57.4	314-343 bp	1.0	66.9
ndhE-ndhG_p2	GGCACTCAAAACAAGTACATGT	AGTTGCCTTGATAGGTGCAA	40.9	45.0	57.4	57.4	324-353 bp	1.0	62.8
ndhE-ndhG_p3	ACAAGTACATGTTCGAGCATCA	AGTTGCCTTGATAGGTGCAAT	40.9	42.9	58.1	57.9	314-343 bp	1.0	62.8
ndhE-ndhG_p4	CAAGTACATGTTCGAGCATCA	AGTTGCCTTGATAGGTGCAA	42.9	45.0	56.4	57.4	313-342 bp	1.0	62.4
ndhE-ndhG_p5	ACAAGTACATGTTCGAGCATC	AGTTGCCTTGATAGGTGCAA	42.9	45.0	56.4	57.4	314-343 bp	1.0	62.4