Hotspot

ccsA-ndhD

SSC · 116127-116404 · 278 bp

Back to Hepatica

Pi 0.0714

MarkerSeek score 72.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 278
variable_sites: 47
pi: 0.0714
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGTATTCTTTCGGGAGCAG	TTGCATTGCCTGGAATGAGT	55.0	45.0	58.0	58.1	833-912 bp	1.0	87.6
ccsA-ndhD_p2	CTAATGAAGCGTGGGGATCC	TTGCATTGCCTGGAATGAGT	55.0	45.0	58.1	58.1	807-886 bp	1.0	87.2
ccsA-ndhD_p3	GAAGCGTGGGGATCCTATTG	TTGCATTGCCTGGAATGAGT	55.0	45.0	58.1	58.1	802-881 bp	1.0	87.2
ccsA-ndhD_p4	GGGTATTCTTTCGGGAGCAG	CATTGCCTGGAATGAGTGGT	55.0	50.0	58.0	58.2	830-909 bp	1.0	87.0
ccsA-ndhD_p5	GGGTATTCTTTCGGGAGCAG	CTTCTCTTGCATTGCCTGGA	55.0	50.0	58.0	58.2	839-918 bp	1.0	86.8