Hotspot

ycf1-ndhF

SSC · 111280-111366 · 87 bp

Back to Hepatica

Pi 0.398

MarkerSeek score 72.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 87
variable_sites: 69
pi: 0.398
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CATATCCGCCCAGTTTGTCA	CAGGTATAAGAAGGCTGGCG	50.0	55.0	58.0	58.1	710-753 bp	0.777778	81.8
ycf1-ndhF_p2	ACATATCCGCCCAGTTTGTC	CAGGTATAAGAAGGCTGGCG	50.0	55.0	58.0	58.1	711-754 bp	0.777778	81.8
ycf1-ndhF_p3	CATATCCGCCCAGTTTGTCA	AAGGCTGGCGGAACTTATTT	50.0	45.0	58.0	57.8	700-743 bp	0.777778	81.1
ycf1-ndhF_p4	ACATATCCGCCCAGTTTGTC	AAGGCTGGCGGAACTTATTT	50.0	45.0	58.0	57.8	701-744 bp	0.777778	81.1
ycf1-ndhF_p5	CATATCCGCCCAGTTTGTCA	GAAGGCTGGCGGAACTTATT	50.0	50.0	58.0	58.2	701-744 bp	0.777778	80.8