Hotspot

petA-psbJ

LSC · 64107-64544 · 438 bp

Back to Hepatica

Pi 0.0156

MarkerSeek score 68.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 19
pi: 0.0156
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCATCTGTTATTCTGGCA	TGGCTTATAGGCACTGTAGC	45.0	50.0	57.2	57.1	617-738 bp	1.0	70.8
petA-psbJ_p2	TGGCATCTGTTATTCTGGCA	AGGCACTGTAGCTGGTATTC	45.0	50.0	57.2	57.0	609-730 bp	1.0	70.2
petA-psbJ_p3	TGGCATCTGTTATTCTGGCA	GGCACTGTAGCTGGTATTCT	45.0	50.0	57.2	57.0	608-729 bp	1.0	70.2
petA-psbJ_p4	TGGCATCTGTTATTCTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.2	58.1	599-720 bp	1.0	69.5
petA-psbJ_p5	TGGCATCTGTTATTCTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.2	57.8	600-721 bp	1.0	68.7