Hotspot

psbM-petN

LSC · 24995-26006 · 1012 bp

Back to Hepatica

Pi 0.00535

MarkerSeek score 61.60

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 19
pi: 0.00535
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.6	58.0	1148-1179 bp	1.0	71.9
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	AGTAAGTCTTGCTTGGGCTG	42.9	50.0	57.6	57.8	1154-1185 bp	1.0	70.4
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	42.9	50.0	57.6	57.6	1094-1125 bp	1.0	69.3
psbM-petN_p4	AAAACGGTCAGTCAAAACGA	TCTTGCTTGGGCTGCTTTAA	40.0	45.0	56.1	58.0	1093-1124 bp	1.0	68.4
psbM-petN_p5	TGCTACTGCACTGTTCATTC	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	56.1	58.0	1148-1179 bp	1.0	67.7