Hotspot

ycf3-psaA

LSC · 11284-12015 · 732 bp

Back to Hepatica

Pi 0.0101

MarkerSeek score 48.70

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 21
pi: 0.0101
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TGAAATTGCAGAGGCTTGGT	ATGCCACTCAGCCAAAGAAA	45.0	45.0	58.0	58.0	1094-1128 bp	1.0	84.0
ycf3-psaA_p2	TGAAATTGCAGAGGCTTGGT	TGCCACTCAGCCAAAGAAAT	45.0	45.0	58.0	58.0	1093-1127 bp	1.0	84.0
ycf3-psaA_p3	TGAAATTGCAGAGGCTTGGT	TAATGGAACCAACCGGCAAA	45.0	45.0	58.0	58.0	1364-1398 bp	1.0	83.2
ycf3-psaA_p4	GTTAGAGGAAGCGCCGTAAA	ATGCCACTCAGCCAAAGAAA	50.0	45.0	58.0	58.0	1651-1689 bp	1.0	82.8
ycf3-psaA_p5	GTTAGAGGAAGCGCCGTAAA	TGCCACTCAGCCAAAGAAAT	50.0	45.0	58.0	58.0	1650-1688 bp	1.0	82.8