Hotspot

trnK-UUU-psbA

LSC · 5201-5546 · 346 bp

Back to Hepatica

Pi 0.0179
MarkerSeek score 59.60
Primer available yes
Label trnK-UUU-psbA

Feature Metadata

label_name
trnK-UUU-psbA
feature_type
igs
region
LSC
length_bp
346
variable_sites
17
pi
0.0179
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.93
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-psbA_p1 ACTCCATCCGACTAGTTCCG TCGCGTCTCTCTAAAATTGCA 55.0 42.9 58.6 58.0 243-414 bp 1.0 74.3
trnK-UUU-psbA_p2 ACTCCATCCGACTAGTTCCG TACCCAGTTACAGAAGCGAC 55.0 50.0 58.6 57.0 280-451 bp 1.0 74.2
trnK-UUU-psbA_p3 ACTCCATCCGACTAGTTCCG CAGTTACAGAAGCGACCCC 55.0 57.9 58.6 57.9 276-447 bp 1.0 73.3
trnK-UUU-psbA_p4 ACTCCATCCGACTAGTTCCG CCAGTTACAGAAGCGACCC 55.0 57.9 58.6 57.9 277-448 bp 1.0 73.3
trnK-UUU-psbA_p5 ACTCCATCCGACTAGTTCCG CCCAGTTACAGAAGCGACC 55.0 57.9 58.6 57.9 278-449 bp 1.0 73.3