Hotspot

rps4

LSC · 48867-49472 · 606 bp

Back to Hemsleya

Pi 0.00248

MarkerSeek score 55.50

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 3
pi: 0.00248
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	GAGGCCAAAATTGTCCGAAC	ACTTTACTGGATTTCGCTTCA	50.0	38.1	57.7	55.5	731 bp	1.0	49.8
rps4_p2	GGAGGCCAAAATTGTCCGAA	ACTTTACTGGATTTCGCTTCA	50.0	38.1	58.7	55.5	732 bp	1.0	46.6
rps4_p3	GAGGCCAAAATTGTCCGAAC	ACTTTACTGGATTTCGCTTCAT	50.0	36.4	57.7	56.1	731 bp	1.0	46.2
rps4_p4	CCAAAATTGTCCGAACCCTTG	ACTTTACTGGATTTCGCTTCA	47.6	38.1	58.0	55.5	727 bp	1.0	44.4
rps4_p5	GCCAAAATTGTCCGAACCCT	ACTTTACTGGATTTCGCTTCA	50.0	38.1	59.0	55.5	728 bp	1.0	44.3