Hotspot

ccsA

SSC · 117298-118269 · 972 bp

Back to Hemsleya

Pi 0.00446

MarkerSeek score 37.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 8
pi: 0.00446
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	1358-1361 bp	1.0	79.7
ccsA_p2	TGAAATCGGTAGACACGCTG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	1357-1360 bp	1.0	79.7
ccsA_p3	GTGAAATCGGTAGACACGCT	TCAGTTGACAAGGTCGAAGC	50.0	50.0	58.0	58.1	1359-1362 bp	1.0	79.7
ccsA_p4	TGAAATCGGTAGACACGCTG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	58.0	58.1	1358-1361 bp	1.0	79.7
ccsA_p5	GAGAAAGCCGCTATGGTGAA	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	1373-1376 bp	1.0	79.6