Hotspot

rpl32-trnL-UAG

SSC · 115916-117101 · 1186 bp

Back to Hemsleya

Pi 0.00604

MarkerSeek score 40.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1186
variable_sites: 14
pi: 0.00604
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAGCAGCGTTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1301-1302 bp	1.0	80.2
rpl32-trnL-UAG_p2	GAGCAGCGTTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1302-1303 bp	1.0	80.2
rpl32-trnL-UAG_p3	GAGCAGCGTTGAAAGCTTTT	CCCTTTTCTGACGAAGCGTA	45.0	50.0	57.9	57.9	1597-1600 bp	1.0	79.8
rpl32-trnL-UAG_p4	GAGCAGCGTTGAAAGCTTTT	AATACAAAATGCCATGCCGC	45.0	45.0	57.9	58.1	1366-1367 bp	1.0	79.7
rpl32-trnL-UAG_p5	GAGCAGCGTTGAAAGCTTTT	GGGGACTAAGAGTGGGGATT	45.0	55.0	57.9	58.1	1859-1862 bp	1.0	79.5