Hotspot

rps16-trnQ-UUG

LSC · 6462-8199 · 1738 bp

Back to Hemsleya

Pi 0.00432
MarkerSeek score 60.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1738
variable_sites
15
pi
0.00432
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1806-1811 bp 1.0 79.2
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1817-1822 bp 1.0 78.7
rps16-trnQ-UUG_p3 CGGTGGGTGTAAGAATCCAC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1843-1848 bp 1.0 78.2
rps16-trnQ-UUG_p4 AACGGTGGGTGTAAGAATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1845-1850 bp 1.0 76.4
rps16-trnQ-UUG_p5 ACGGTGGGTGTAAGAATCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 1844-1849 bp 1.0 75.1