Hotspot

rps16

LSC · 4869-6053 · 1185 bp

Back to Hemipilia

Pi 0.0231

MarkerSeek score 48.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1185
variable_sites: 41
pi: 0.0231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TATTTGTTCGAGACGCCGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1925-2041 bp	1.0	85.1
rps16_p2	GGATCAGTCGCGGTATTCTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	57.9	58.1	2336-2468 bp	1.0	84.8
rps16_p3	GTTGAGTTAGCAACCCGGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	2234-2366 bp	1.0	84.6
rps16_p4	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2252-2384 bp	1.0	84.1
rps16_p5	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2251-2383 bp	1.0	84.1