Hotspot

rpl32-trnL-UAG

SSC · 111835-112471 · 637 bp

Back to Hemipilia

Pi 0.0346

MarkerSeek score 72.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 637
variable_sites: 33
pi: 0.0346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGTTGCAATGAGCTGGTCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1369-1426 bp	1.0	86.8
rpl32-trnL-UAG_p2	AGTTGCAATGAGCTGGTCAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	1362-1419 bp	1.0	86.3
rpl32-trnL-UAG_p3	AGTTGCAATGAGCTGGTCAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.0	57.8	1368-1425 bp	1.0	86.2
rpl32-trnL-UAG_p4	AGTTGCAATGAGCTGGTCAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.0	57.8	1367-1424 bp	1.0	86.2
rpl32-trnL-UAG_p5	CTTTTTGACTGTCCGGTGGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	809-811 bp	1.0	84.4