Hotspot

petA-psbJ

LSC · 61067-61865 · 799 bp

Back to Hemipilia

Pi 0.0217

MarkerSeek score 64.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 26
pi: 0.0217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	TATTCCGGGTTGGGTTCATC	38.1	50.0	55.4	57.3	846-860 bp	1.0	49.3
petA-psbJ_p2	TGAAAAGGTTCAGTTGTACGA	CTATTCCGGGTTGGGTTCAT	38.1	50.0	55.4	57.3	847-861 bp	1.0	49.2
petA-psbJ_p3	ACAGTTTGAAAAGGTTCAGTTGT	TATTCCGGGTTGGGTTCATC	34.8	50.0	57.3	57.3	852-866 bp	1.0	48.2
petA-psbJ_p4	ACAGTTTGAAAAGGTTCAGTTGT	CTATTCCGGGTTGGGTTCAT	34.8	50.0	57.3	57.3	853-867 bp	1.0	48.1
petA-psbJ_p5	AGGTTCAGTTGTACGAAATGA	TATTCCGGGTTGGGTTCATC	38.1	50.0	55.2	57.3	841-855 bp	1.0	47.5