Hotspot

accD-psaI

LSC · 57075-57735 · 661 bp

Back to Hemipilia

Pi 0.0363

MarkerSeek score 81.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 34
pi: 0.0363
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCGGCTGAGTATTCATTCCA	AGAAACCATTGCAATTGCCG	50.0	45.0	58.0	57.9	848-857 bp	1.0	83.8
accD-psaI_p2	GCGGCTGAGTATTCATTCCA	TGCAATTGCCGGAAAGACTA	50.0	45.0	58.0	57.8	839-848 bp	1.0	83.0
accD-psaI_p3	GCGGCTGAGTATTCATTCCA	CATTGCAATTGCCGGAAAGA	50.0	45.0	58.0	57.6	842-851 bp	1.0	81.6
accD-psaI_p4	GCGGCTGAGTATTCATTCCA	ATTGCAATTGCCGGAAAGAC	50.0	45.0	58.0	57.6	841-850 bp	1.0	81.6
accD-psaI_p5	GCGGCTGAGTATTCATTCCA	CGGAAAGACTAGGCCCACTA	50.0	55.0	58.0	58.5	830-839 bp	1.0	80.5